Rat and mouse models, male/female, pure compounds or complex mixtures/extracts,

the breeding certificate for the animals is 67149/2018-MZE-17214,

safety assessment after drug candidate/natural product treatment, in line with OECD standards,

external inspection (photo documentation) and autopsy,

collection of organs (gingival tissue, all including the brain) and body fluids (blood, urine),

hematology, red blood count, white blood count,

basic blood and urine biochemistry (substrates, enzymes, ions, lipids), urinalysis, accreditation according to EN ISO 15189: 2013,

oxidative stress parameters, genotoxicity and hepatotoxicity in vivo,

histology of organs, basic examination, special examination (staining, e.g. for collagen),

immunohistochemistry of selected (target) proteins,

continual monitoring of animals, including recording of weight (if necessary, recording of feed water and feed consumption),

continuous collection of urine/stool/small blood sample (up to 10 uL),

administration route (per os, per rectum, i.v., i.m., s.c., special probes),

Irwin test and functional observational battery,

implantation of tumors (via tumor cell lines),

small operations with survival of the animal,

pharmacokinetics, ADME, NOEL, metabolic studies by using already established LC-MS profiling based on GMP Certificate Ref. No.: sukls223661/2021,

complex behavioral and neurological in vivo testing.